Neutralization of tier-2 viruses and epitope profiling of plasma antibodies from subtype-C HIV-1 infected north Indians; implications for MPER directed HIV-1 neutralization
نویسندگان
چکیده
Methods Plasma of eighty naïve HIV-1 seropositive Indian patients were tested for neutralization against a panel of 3 subtype-B and 5 subtype-C tier 1 and tier 2 viruses. Three plasma, found to be broadly neutralizing (bNP), were mapped with a set of 212 consensus-C gp160 overlapping peptides (15mer each). 50% binding titers were determined by ELISA using consensus-C V3 (35mer), IDR loop (19mer) and MPER (24mer) peptides.
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